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Urine samples and standards were transferred to 96-well plates, taxifolin (1 g/mL) was added as internal standard, and samples were vortexed and centrifuged at maximum speed for 2 min before LC-MS/MS analysis

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We have quite a few additional articles discussing glutathione agarose beads and GST affinity purification, and you can find links to those at the end of the article

Assessing the human gut microbiota in metabolic diseases

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