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We chose (EAAAK) 3 rigid helical linkers to work because we believe their rigidity allows for the separation of the protein domains and improves the functionality of individual domains 58,59 when compared to flexible linkers, which appear to be suitable for linking normally connected domains in a single genetic construct, such as antibody domains in scFvs 60

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Multiplex KRASG12/G13 Mutation Testing of Unamplified Cell-Free DNA from the Plasma of Patients with Advanced Cancers Using Droplet Digital Polymerase Chain Reaction