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Reproductive toxicity studies in animal models have reported altered sperm parameters and effects on estrous cyclicity at supra-therapeutic doses (92)
Frontiers In Immunology, 8, 677
Briefly, 100120 islets were washed 2 times using modified HBSS, then dispersed in 600 L Accutase (Innovative Cell Technologies, catalog AT-104) by gently pipetting up and down for 7 minutes at 37C
If there is any doubt about the integrity of a prepared solution, it must be discarded
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