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Western blot analysis Heart tissues or cells from mice were lysed with lysis buffer (20 mM TrisHCl, pH 7.4

These stressors create free radicals

Pan W, Jiang P, Zhao J, Shi H, Zhang P, Yang X, et al

The elucidation of arGSTs role as biosynthetic enzymes in the anthocyanin pathway has a direct implication for production of these important pigments in microbial cell factories, as highlighted by a 36.5-fold improvement of the cyanidin-3- O -glucoside ( 13 ) titre through the coexpression of arGSTs in our prototype S

We hypothesize that the influence of SFN on the gut microbiota is at least partly related to the effects on their habitat, i.e., the gut such as (i) alleviation of oxidative stress and inflammation in the intestine, (ii) increase of the expression of tight junction proteins, preventing excessive intestinal permeability and invasion of luminal bacteria, and (iii) promotion of gut detoxification capability via increase of activity of phase II detoxification enzymes such as GSTs

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