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In vitro studies have documented 40-70% reductions in pro-inflammatory cytokine production, including TNF-alpha, IL-1beta, and IL-6, when inflammatory cells are pre-treated with GHK-Cu (1-5 M) prior to activation with lipopolysaccharide or other inflammatory stimuli
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An additional indicative point is sequential involvement
This is due to their potential for some serious side effects, such as an allergy, although they occur very rarely
Once reconstituted, the solution should be used promptly or aliquoted and stored at -20C to avoid repeated freeze-thaw cycles, which can degrade the compound
