Though G6PD is found in the cytoplasm of all mammals cells, its deficiency is more evident in the red blood cells probably because these cells live without nucleus for a long time and because they contain proteases that degrade the mutant enzyme in major degree than other proteases in other tissues 1
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Am J Physiol Cell Physiol 302 (12), C1741C1750
See how long does BPC-157 take to work for the underlying timeline
Briefly, frozen sections were treated with basic ethanol (80% ethanol/1% NaOH) for 5 min, and washed in 70% EtOH and ultrapure water for 2 min, respectively