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For glutathione and glutamate quantification, cell viability analysis and oxidative stress assay, cells were sub-cultured in 12-well plates at a density of 4.2 10 5 cells/well
A recent meta-analysis found no link between GLP-1 agonists and pancreatitis in people with Type 2 diabetes, but some case reports challenge this
Cells were cultured in complete RPMI 1640/GlutaMAX medium (GIBCO, 61870-010) supplemented with 10% heat-inactivated human AB serum (Institut Jacques Boy, 201021334), 1% penicillinstreptomycin (GIBCO, 15140-122), 1% HEPES (GIBCO, 15630-056), 1% sodium pyruvate (GIBCO, 11360-039) and 1% polymyxin B (final concentration 10 g ml 1
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Semaglutide is the active ingredient in two FDA-approved drugs: Ozempic and Wegovy
360 mg Chlorine (chloride) 14720 mg Sodium 8000 mg Potassium
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