To prevent precipitation CBS FL-CHis and its mutants were buffer exchanged into 20 mM HEPES, pH 7.4, 0.01% triton X-100 whereas CBS RD was exchanged into 20 mM HEPES, pH 7.4, 200 mM NaCl
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placebo), most of them (liraglutide, semaglutide SC, dulaglutide, albiglutide, and efpeglenatide) have also demonstrated a reduction in MACE with respect to placebo, while weekly exenatide or oral semaglutide showed a tendency to reduce MACE
SURPASS 1, SURPASS 5, and SURPASS J-combo are placebo controlled, while others have active comparators: for example GLP-1RAs (dulaglutide and semaglutide), long-acting insulin analogues (glargine and degludec), or short-acting insulin analogue (lispro)