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Binding/Wash Buffer: PBS, pH 7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.4) Elution Buffer: 50 mM Tris-HCl, 10-20 mM reduced glutathione, pH8.0 2.2 Preparation of the Magnetic Beads 1) Completely resuspend the beads by shaking or vortexing the vial

Antonoff MB, Mitzman B, Backhus L, Bradbury ST, Chatterjee S, Cooke DT, Crestanello J, Goldstone AB, Kim KM, Nguyen TC, Romano JC, Vaporciyan AA, Varghese TK

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In order to verify the safety/low toxicity of the employed concentrations, we examined the possible apoptotic effects of ALCAR on peripheral blood mononuclear cells (PBMCs) from healthy donors

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