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Studied for cell growth, repair, and anabolic signaling
Introduction The first reports about a plant glutathione S-transferase enzyme (GST, EC 2.5.1.18) appeared in 1970, when it was revealed that a GST catalyzed the detoxification of the herbicide atrazine by its conjugation to the endogenous tripeptide glutathione (GSH, -L-glutamyl-L-cysteinyl-glycine) in sorghum and maize plants (Frear and Swanson, 1970)
A 96-well plate was used, where 10 L of the sample was mixed with 180 L of glycine buffer at 32 C, followed by 5 L of catalase