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Activated and functional autophagy is linked with life longevity (Cant and Auwerx 2009)
After achieving a sufficient level of narcosis, typically within 2 min, mice were placed in a supine position on a temperature-controlled self-regulated heating system calibrated to 38 C and fixed with stripes at all extremities
Sudden cooling and thawing three times cause proteins of erythrocytes membrane to denature and eventually lead to cell lysis
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Critically, these effects were absent in 3-AR knockout mice, indicating that an intact 3-AR pathway is required for the lipolytic activity
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